(YL-CN1) Electroporation

(YL-CN1) Electroporation 1).Electroporation 2).Mesotherapy 3).Beauty Equipment 4).Beaut Life

(YL-CN1) Electroporation

1).Electroporation2).Mesotherapy3).Beauty Equipment4).Beaut Life

Electroporation Working Theory

Electroporation is a mechanical method used to introduce polar molecules into a host cell through the cell membrane. In this procedure, a large electric pulse temporarily disturbs the phospholipid bilayer, allowing molecules like DNA to pass into the cell.

  

Applications

As previously mentioned, electroporation is widely used in many areas of molecular biology research and in the medical field. Some applications of electroporation include:

- DNA Transfection or Transformation: This is likely the most widespread use of electroporation. Specific genes can be cloned into a plasmid and then this plasmid introduced into host cells (bacterial or otherwise) in order to investigate gene and protein structure and function.

 

 Figure 5. Microscope images of the results of transfection by electroporation. In this experiment, a gene construct was inserted by electroporation into the cells shown on the right. The fluorescence of the protein produced by the reporter gene included in this construct shows that the DNA was properly uptaken in the majority of cells. These cells could now be used in further experimentation.

- Direct Transfer of Plasmids Between Cells: Bacterial cells already containing a plasmid may be incubated with another strain that does not contain plasmids but that has some other desireable feature. The voltage of electroporation will create pores, allowing some plasmids to exit one cell and enter another. The desired cells may then be selected by antibiotic resistance or another similar method. This type of transfer may also be performed between species. Thus, large numbers of plasmids may be grown in rapidly multiplying bacterial colonies and then transferred to yeast cells by electroporation for study.

- Induced Cell Fusion: The disruption of the membrane that occurs with the quick pulse of electricity in the electroporation procedure has also been shown to induce fusion of cells.

- Trans-dermal Drug Delivery: Just as electroporation causes temporary pores to form in plasma membranes, studies suggest that similar pores form in lipid bilayers of the stratum corneum- the outermost dead layer of skin. These pores could allow drugs to pass through to the skin to a target tissue.

  

- Gene Therapy: Much like drug delivery, electroporation techniques can allow vectors containing important genes to be transported across the skin and into the target tissue. Once incorporated into the cells of the body, the protein produced from this gene could replace a defective one and thus treat a genetic disorder (Figure 6).

 

Figure 6. Diagram of the method of gene therapy using electroporation.

Illustration of the machine

 Illustration of Operating Panel

 Illustration of LCD Display

How to Use

Plug in the power code into the power socket.Plug in the treatment cable, and connect the treatment probe and Anode plate.

(Note: there are two treatment probes one is big, the other is small, according to the treatment area to choose the suitable treatment probe.)

Press the “Power On/off” button to start the machine.Press the button up and down key to choose the suitable treatment time.Press the “Right” button, and then press up and down key to choose the suitable treatment intensity.Press “Start/pause” button to start the function of Electroporation.After treatment, please press “Power On/off” button to switch off the power.

Preparation of Treatment

Clean the treatment parts first by clean lotion or by diamond dermabrasion.Clean the treatment probe by Alcohol, if possible put the probe into the UV sterilizer for 15 minutes. Put the cosmetics product into the bottle of the treatment probe.

How to maintenance 

Please clean the treatment probe and the Anode plate after treatment.Plug out the power code from the power socket.Keep the machine in a dry and clean place.

Technical parameters. 

Power Input: 110V/60Hz or 220V/50Hz

Max. Power: 15 W

Temperature range: 10°C-42°C

 

Accessories 

Main unit 1 pc

Treatment probe 2 pcs

Anode plate 1 pc

Treatment cable 1 pc

Power Cord 1 Pc

Manual 1 pc

Fuse 2 pc

Paper Carton 1 pc

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(YL-CN1) Electroporation